Ribonuclease A.
نویسنده
چکیده
منابع مشابه
Isolation and characterization of ovine ribonuclease A, B, and C from pancreatic secretion.
Chromatography over columns of CM-cellulose and Amberlite IRC-50 was used to fractionate the ribonucleases present in the pancreatic secretion of sheep. The chromatographic behavior of these enzymes was similar to that of the enzymes found in bovine pancreatic secretion, as was also their relative distribution. The principal component, ribonuclease A, accounted for -85% of the ribonuclease in t...
متن کاملRibonuclease A digestion by proteinase K.
The digestion of ribonuclease A by proteinase K yielded one major degradation product only, which could not be distinguished from ribonuclease S by electrophoretical and immunological methods. This component (ribonuclease K) possessing full catalytic activity was characterized to be (1--20/21--124) ribonuclease A. Combined action of proteinase K and trypsin on ribonuclease A leads to a signific...
متن کاملRibonuclease Activity in Some Transplantable Rat Hepatomas.
Several parameters of ribonuclease activity have been determined in a series of transplantable rat tumors and liver from the host rats. These include ribonuclease inhibitor activity, pH activity curves, and the distribution and specific activity of acid and alkaline ribonuclease in subcellular fractions. For those hepatomas studied, compared with liver from the tumor-bearing animal, the followi...
متن کاملA Practical Preparation of Ribonuclease-S and the Action of Subtilisin on Ribonuclease-B ‡
All preparations of ribonuclease-S so far reported have employed limited digestion of ribonuclease-A with subtilisin,' a bacterial proteinase from B. subtilis described by Giintelberg and Ottesen.' The original supply of this latter enzyme is now exhausted. Another sample of a crystalline proteinase from B. subtilis (NOVO Enzyme) has been shown to differ from the original in several respects,' ...
متن کاملCrystalline semisynthetic ribonuclease-S'.
Crystals of solid phase-derived semisynthetic ribonuclease-S' were prepared and compared with those for native ribonuclease-S' and -S. The semisynthetic species used was the noncovalent complex of synthetic fragment-(1-20), corresponding to residues 1 through 20 of bovine pancreatic ribonuclease-A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22), and native ribonuclease-S-(21-...
متن کاملThe Ribonuclease P Database
Ribonuclease P is the endoribonuclease responsible for the removal of leader sequences from tRNA precursors. Ribonuclease P is a ribonucleoprotein, and in bacteria the RNA alone is capable of pre-tRNA processing in vitro, i.e. it is a catalytic RNA. The Ribonuclease P Database is a compilation of ribonuclease P sequences, sequence alignments, secondary structures, three-dimensional models and a...
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ورودعنوان ژورنال:
- Chemical reviews
دوره 98 3 شماره
صفحات -
تاریخ انتشار 1998